This competition will be used to fill two positions.
This position has a term length of one year with the possibility of extension based on the availability of funding.
The Division of Surgical Research within the University of Alberta conducts research throughout a range of investigation from molecular and cell biology, to translational and educational research, to multicenter randomized clinical trials. The Surgical Research team supports and promotes discovery and clinical research with a focus on developing the research abilities of trainees and fostering the synergistic potential of collaboration between basic scientists and clinician scientists. The Division supports the mission of the Department to restore health through surgical intervention, remembering patient dignity and comfort are integral to overall health, and is committed to excellence in creating important knowledge and better treatments for patients. Further information on the Department of Surgery and Division of Surgical Research can be found at www.ualberta.ca/surgery.
Reporting to the Principal Investigators (PI), Dr. Gregory Korbutt, Professor, and Dr. Andrew Pepper, Assistant Professor, the Laboratory Technician will assist with the design, implementation, and analysis of diabetes and transplant related research projects. A major component of this role will involve tissue culture of small and large animal models as well as human cells.
Assist with daily laboratory activities such as maintenance of lab equipment, purchasing supplies, maintaining all inventories, doing dishes, autoclaving lab supplies, preparing solutions, general cleaning
Assist in sample processing and animal model experiments involving mouse, rat, pig, and human cell cultures and transplants in collaboration with the PI
Participate in animal surgeries and post-operative monitoring
Plan and develop experimental protocols in collaboration with the PI, graduate/summer students, postdocs, trainees and technical staff
Conduct genetic manipulation of mouse cell lines (CRISPR/CAS9)
Complete data collection including analyzing tissue samples for oxygen consumption rates, insulin secretion and content, DNA, glucagon, cytokine profiles, and cell death markers using flow cytometry, fluorometric, ELISA and colorimetric assays
Conduct immunohistochemistry on cell samples, organs and transplant specimens
Collection and interpretation of experimental data to determine relevant significance and eventual publications
Train other staff, students, and visiting investigators in technologies and techniques developed in the laboratory
Recognize and react to biosafety and chemical safety in the lab, ensuring proper handling and treatment of hazardous materials
Minimum post-secondary diploma in Bioscience Technology or a related field
Minimum 2 years of experience in a laboratory setting
Experience in cell culture will be considered a strong asset
Experience with handling and care of lab animals, use of small animal surgical equipment, and animal handling certification will be considered an asset
Computer proficiency with MS Office (Word, Excel, PowerPoint) and Google Apps (Gmail, Google Calendar, Google Docs)
Strong analytical skills with the ability to collect, organize, analyze, and disseminate information with attention to detail and accuracy
Strong interpersonal and communication skills both written and verbal
Highly developed organizational and time management skills including demonstrated ability to manage multiple deadlines and projects